Puromycin dihydrochloride BioChemica

Assay (HPLC): min. 98 %

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code	packaging size	price per unit	box price per unit
Code & packaging		Price per piece
code A2856,0010	packaging size 10 mg	price per unit single 30,60€	box price per unit
code A2856,0025	packaging size 25 mg	price per unit single 69,90€	box price per unit
code A2856,0100	packaging size 100 mg	price per unit single 225,00€	box price per unit

Product Code:: A2856
Product Name:: Puromycin dihydrochloride BioChemica
Specifications:: Assay (HPLC): min. 98 %
IR-Spectrum: passes test
Solubility (5 %; H₂O): clear, colorless
Comment:: Puromycin is an aminonucleoside antibiotic from Streptomyces alboniger. It is an analogon to aminoacyl-tRNA and inhibits the protein synthesis by termination of the peptidyl transfer at the ribosomes in prokaryotes and eukaryotes. The antibiotic inhibits the growth of gram positive bacteria and different animal cells. Fungi and gram negative bacterias are resistant, since puromycin cannot pass the cell wall. The discovery of the puromycin-N-acetyltransferase (PAC) in a Streptomyces-stem allowed to employ the antibiotic as a selection marker, comparable to neomycin. The working concentration is for mammalian cells depending on the cell line between 1 and 50 μg/ml.Stock solutions of puromycin (10 mg/ml) can be prepared in HEPES buffer and are stored at \+4°C or -20°C (improves stability) up to one year.
Bibliography:: (1)Sambrook, J. & Russell, D.W. (2001) Molecular Cloning\: A Laboratory Manual, 3rd Edition Page 16.49 \+ 17.65. CSHL Press, Cold Spring Harbor, NY. (2)De La Luna, S. & Ortin, J. (1992) Methods Enzymol. 216, 376-385pac-Gen as Efficient Dominant Marker and Reporter Gene in Mammalian Cells.