Packs sizes (3)
code | packaging size | price per unit | box price per unit | |
---|---|---|---|---|
Code & packaging | Price per piece | |||
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code
A2856,0010
|
packaging size
10 mg
|
price per unit
single
30,60€
|
box price per unit
|
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code
A2856,0025
|
packaging size
25 mg
|
price per unit
single
69,90€
|
box price per unit
|
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code
A2856,0100
|
packaging size
100 mg
|
price per unit
single
225,00€
|
box price per unit
|
Technical data
- Physical Description:
- Solid
- Product Code:
- A2856
- Product Name:
- Puromycin dihydrochloride BioChemica
- Specifications:
- Assay (HPLC): min. 98 %
IR-Spectrum: passes test
Solubility (5 %; H2O): clear, colorless
- Comment:
Puromycin is an aminonucleoside antibiotic from Streptomyces alboniger. It is an analogon to aminoacyl-tRNA and inhibits the protein synthesis by termination of the peptidyl transfer at the ribosomes in prokaryotes and eukaryotes. The antibiotic inhibits the growth of gram positive bacteria and different animal cells. Fungi and gram negative bacterias are resistant, since puromycin cannot pass the cell wall. The discovery of the puromycin-N-acetyltransferase (PAC) in a Streptomyces-stem allowed to employ the antibiotic as a selection marker, comparable to neomycin. The working concentration is for mammalian cells depending on the cell line between 1 and 50 μg/ml. Stock solutions of puromycin (10 mg/ml) can be prepared in HEPES buffer and are stored at \+4°C or -20°C (improves stability) up to one year.
- Bibliography:
(1) Sambrook, J. & Russell, D.W. (2001) Molecular Cloning\: A Laboratory Manual, 3rd Edition Page 16.49 \+ 17.65. CSHL Press, Cold Spring Harbor, NY. (2) De La Luna, S. & Ortin, J. (1992) Methods Enzymol. 216, 376-385 pac-Gen as Efficient Dominant Marker and Reporter Gene in Mammalian Cells.
- Hazard pictograms
-
- WGK:
- 1
- Storage:
- -20°C
- Signal Word:
- Attention
- GHS Symbols:
- GHS07
- H Phrases:
- H302
- P Phrases:
- P264
P270
P301+P312
- EINECS:
- 200-387-8
- CS:
- 29419000