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Bicine for buffer solutions

Assay (titr.): min. 99 %
Code
A1024
CAS
150-25-4
Molecular Formula
C6H13NO4
Molar mass
163.17 g/mol

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Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A1024,0250
code
A1024,0250
packaging size
250 g
price per unit
single 101,50€
box price per unit
Solubility:
100 g/L (H2O)
Physical Description:
Solid
Product Code:
A1024
Product Name:
Bicine for buffer solutions
Specifications:
Assay (titr.): min. 99 %
pH (1 %; H2O; 20°C): 4.0 - 5.0
Heavy metals (as Pb): max. 0.0005 %
Water: max. 1 %
A (1 cm/0.1 M in H2O)
260 nm: max. 0.08
280 nm: max. 0.05
Comment:
Bicine is a versatile buffer. Its applications last from enzyme reaction buffers (T4 polynucleotide kinase ref. 4; Micrococcus nuclease ref. 5; adenylate cyclase ref. 6) to electrophoresis buffers (3). Bicine interferes with the BCA and Lowry protein assay. Ferricyanide slowly oxidizes bicine. In addition, its strong binding of copper ions may be disturbing. The working concentration lasts from 3 to 100 mM.
Bibliography:
(1)Good, N.E. et al. (1966) Biochemistry 5, 467-477Hydrogen ion buffers for biological research. (2)Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68Hydrogen ion buffers. (3)Werb, Z. et al. (1974) Biochem. J. 139, 359-368Interaction of α2-Macroglobulin with Proteinases. (4)Vodicka, P. et al. (1995) Carcinogenesis 16, 1473-1481DNA damage by styrene. (5)Eide, I. et al. (1995) Carcinogenesis 16, 1603-1609Uptake of hemoglobin and DNA adducts after inhalation of C2-C8 1-Alkanes (Olefines) in rats. (6)Peterkofsky, A. et al. (1995) Biochemistry 34, 8950-8959The E. coli adenylate cyclase complex\: Requirements of the PTS protein for the stimulation by nucleotides.
WGK:
1
Storage:
RT
EINECS:
205-755-1
CS:
29225000
Download TDS file for complete specifications