CTAB - Lysis buffer BioChemica

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code	packaging size	price per unit	box price per unit
Code & packaging		Price per piece
code A4150,0500	packaging size 500 ml	price per unit single 27,60€	box price per unit
code A4150,1000	packaging size 1 L	price per unit single 48,60€	box price per unit

Product Code:: A4150
Product Name:: CTAB - Lysis buffer BioChemica
Specifications:: pH (20°C): 8.0 ± 0.1
Composition:
CTAB: 20.00 g/L (2 % w/v)
EDTA · Na₂ · 2H₂O: 7.44 g/L (20 mM)
Tris ultrapure: 12.11 g/L (100 mM)
Sodium chloride: 81.82 g/L (1.4 M)
Comment:: The cationic detergent cetyltrimethylammonium bromide (CTAB) is used to liberate and complex with total cellular nucleic acids. CTAB forms an insoluble complex with nucleic acids when the initial NaCl concentration is lowered to ~0.5 M. Polysaccharides, phenolic compounds and other enzyme-inhibiting contaminats found in plant cells are efficiently removed in the supernatant because most do not precipitate under these conditions (1).
Bibliography:: (1)Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (2001) Current Protocols in Molecular Biology. Page 2.3.5 (Suppl. 45) Greene Publishing & Wiley-Interscience, New York.