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D(-)-Sorbitol (Ph. Eur., USP-NF) pure, pharma grade

Assay (HPLC, dried subst.): 97.0 - 100.5 %
Code
A2222
CAS
50-70-4
Molecular Formula
C6H14O6
Molar mass
182.18 g/mol
Solubility:
2350 g/L (H2O)
Physical Description:
Solid
Product Code:
A2222
Product Name:
D(-)-Sorbitol (Ph. Eur., USP-NF) pure, pharma grade
Specifications:
Assay (HPLC, dried subst.): 97.0 - 100.5 %
Appearance of solution: passes test
Identity: passes test
Red. sugars: max. 0.2 %
Sulfated ash: max. 0.1 %
Total aerobic microbial count (TAMC): max. 1000 CFU/g
Colony count (yeasts & moulds) (TYMC): max. 100 CFU/g
pH (10 %; H2O): 3.5 - 7.0
Conductivity (20 %; H2O dest.): max. 20 μS/cm
E. coli: negative
Salmonella: negative
Water (K.F.): max. 1.5 %
Ni: max. 0.0001 %
Related subst. (HPLC)
Each individual impurity: max. 2.0 %
Total impurities: max. 3.0 %
WGK:
1
Storage:
RT
EINECS:
200-061-5
CS:
29054491
Download TDS file for complete specifications

Comments

Sorbitol is used in high concentration for cell disruption. The stabilizing buffer for enzymatic lysis of yeast contains 1 M Sorbitol (1). Concentrations of 300 mM and 400 mM, respectively, are used for the extraction of chloroplasts and thylacoids, respectively (2).

Literature

(1) Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition; Page 18.36, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. (2) Gegenheimer, P. (1990) Methods Enzymol. 182, 174-193. Preparation of extracts from plants.