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DAPI BioChemica

Assay (HPLC): min. 98 %
Code
A1001
CAS
28718-90-3
Molecular Formula
C16H15N5 · 2HCl
Molar mass
350.25 g/mol

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Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A1001,0010
code
A1001,0010
packaging size
10 mg
price per unit
single $63,30
box price per unit
$53,81x 6 units
A1001,0025
code
A1001,0025
packaging size
25 mg
price per unit
single $117,45
box price per unit
A1001,0100
code
A1001,0100
packaging size
100 mg
price per unit
single $294,60
box price per unit
A1001,0500
code
A1001,0500
packaging size
500 mg
price per unit box price per unit
A1001,9001
code
A1001,9001
packaging size
1 g
price per unit box price per unit
A1001,9010
code
A1001,9010
packaging size
10 g
price per unit box price per unit
Physical Description:
Solid
Product Code:
A1001
Product Name:
DAPI BioChemica
Specifications:
Assay (HPLC): min. 98 %
Solubility (1 %; H2O): clear
N: min. 18 %
UV spectrum
λmax.: 223 nm, 261 nm, 342 nm
λmin.: 246 nm, 282 nm
Comment:
DAPI is an excellent dye for the staining of DNA. Originally, only the specific binding to AT-base pairs without an intercalation was known (2), but later on, the intercalation into GC-base pairs was shown (3). The most popular application of DAPI is its use as a reagent to detect mycoplasma or virus DNA (e. g. vaccinia infection or 'unwanted' viral contamination of cell culture cells) in the cell culture.AppliChem recommends the following simple procedure: Grow cells on a coverslip in a cell culture dish to reach approx. 70 % confluence. Pour off the medium from the cells. Wash the coverslip once with 1 μg/ml DAPI in methanol. Incubate the cells on the coverslip at 37°C for 15 minutes in 1 μg DAPI/ml in methanol. Pour off the staining solution and wash the coverslip once with methanol. Put it up-side-down on a slide with PBS or glycerol as mounting medium. Do not use water. Examine the cells under a microscope (excitation: 365 nm; emission maximum at 450 nm). Prolonged incubation with DAPI increases the nuclear fluorescence, shorter incubation time leads to a weaker nuclear staining, which facilitates the examination of the cytoplasmic fluorescence.Solubility / Stability: Dissolve DAPI in double-distilled water to a final concentration of 1 - 5 mg. The maximum solubility in water is approx. 25 mg/ml. DAPI is insoluble in PBS. Do not use any buffers. Dilute the stock solution with methanol to a final concentration of 1 μg/ml. Solutions are stable at room temperature for 1 - 2 weeks (4), at \+4°C up to 6 months and frozen between 6 and 12 months (1 ml aliquots). If the solution becomes turbid, DAPI is hydrolyzed. DAPI bleaches quickly in contact with light, even if it is quite stable against UV-light. Incubate your samples in the dark. If your samples are stored at \+4°C for one day, fluorescence is stabilzed.
Bibliography:
(1)Russel, W.C. et al. (1975) Nature 253, 461-462A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. (2)Kapuscinski, J. & Szer, W. (1979) Nucleic Acids Res. 6, 3519-3534Interaction of 4',6-diamidine-2-phenylindole with synthetic polynucleotides. (3)Wilson, W.D. et al. (1989) J. Am. Chem. Soc. 111, 5008-5010Binding of 4',6-Diamidino-2-phenylindole (DAPI) to GC and mixed sequences in DNA\: Intercalation of a classical groove-binding molecule. (4)Otto, F. (1990) Methods Cell Biol. 33, 105-110DAPI-staining of cells fixed for "flow cytometry". (5)Schweizer, D. (1976) Chromosoma 58, 307-324'Reverse Fluorescent Chromosome Banding' with Chromomycin and DAPI. (6)Naimski, P. et al. (1980) Anal. Biochem. 106, 471-475Quantitative Fluoreszenzanalyse verschiedener Konformationen von DAPI-gebundener DNA. (7)Daxhelet, G.A. et al. (1989) Anal. Biochem. 179, 401-403Spectrofluorometry of Dyes with DNAs of Different Base Composition and Conformation. (8)Xia, H. et al. (1997) BioTechniques 22, 934-936Detection of Mycoplasma infections of mammalian cells.
WGK:
1
Storage:
2-8°C
protected from light
EINECS:
249-186-7
CS:
29339980
Download TDS file for complete specifications