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Guanidine Hydrochloride BioChemica

Assay (titr.): min. 99 %
Code
A1499
CAS
50-01-1
Molecular Formula
CH5N3 · HCl
Molar mass
95.53 g/mol

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Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A1499,0250
code
A1499,0250
packaging size
250 g
price per unit
single $61,05
box price per unit
A1499,1000
code
A1499,1000
packaging size
1 kg
price per unit
single $153,30
box price per unit
$130,31x 6 units
A1499,5000
code
A1499,5000
packaging size
5 kg
price per unit box price per unit
A1499,9020
code
A1499,9020
packaging size
20 kg
price per unit box price per unit
A1499,9025
code
A1499,9025
packaging size
25 kg
price per unit box price per unit
Solubility:
2000 g/L (H2O)
Physical Description:
Solid
Product Code:
A1499
Product Name:
Guanidine Hydrochloride BioChemica
Specifications:
Assay (titr.): min. 99 %
pH (5 %; H2O; 25°C): 4.0 - 6.0
Insoluble matter: passes test
Water: max. 0.5 %
Ammonium: max. 0.05 %
Fe: max. 0.0005 %
Pb: max. 0.0005 %
A (1 cm/50 % in water HPLC grade)
260 nm: max. 0.1
280 nm: max. 0.05
Comment:
Guanidine hydrochloride denatures proteins and inhibits nucleases. It is applied at high concentrations for the isolation of non-degraded RNA (e. g. stock solution 7.5 M; ref. 1). β-Mercaptoethanol or DTT increase the denaturing activity of guanidine hydrochloride, since they break intramolecular disulfide bonds. Guanidine thiocyanate acts even stronger than guanidine hydrochloride as a denaturant and is used for 'valuable' samples/assays. Strongly hydrophobic proteins, like the membrane protein lipophilin will not be denatured by guanidine hydrochloride (2).
Bibliography:
(1)MacDonald, R.J. et al. (1987) Methods Enzymol. 152, 219-227Isolation of RNA using guanidinium salts. (2)Cockle, S.A. et al. (1978) J. Biol. Chem. 253, 8019-8026Resistance of lipophilin, a hydrophobic myelin protein, to denaturation by urea and guanidinium salts.
Hazard pictograms
  • GHS07 Hazard
WGK:
1
Storage:
RT
Signal Word:
Attention
GHS Symbols:
GHS07
H Phrases:
H302+H332
H315
H319
P Phrases:
P261
P280
P302+P352
P304+P340
P305+P351+P338
EINECS:
200-002-3
CS:
29252900
Index Nr.:
607-148-00-0
Download TDS file for complete specifications