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Propidium Iodide BioChemica

Assay (HPLC): min. 94 %
Code
A2261
CAS
25535-16-4
Molecular Formula
C27H34I2N4
Molar mass
668.40 g/mol

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Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A2261,0025
code
A2261,0025
packaging size
25 mg
price per unit
single $78,90
box price per unit
A2261,0100
code
A2261,0100
packaging size
100 mg
price per unit
single $262,65
box price per unit
A2261,9001
code
A2261,9001
packaging size
1 g
Product discontinued. Alternative A2261,0025 (please check specifications).
A2261,9005
code
A2261,9005
packaging size
5 g
Product discontinued. Alternative A2261,0025 (please check specifications).
Physical Description:
Solid
Product Code:
A2261
Product Name:
Propidium Iodide BioChemica
Specifications:
Assay (HPLC): min. 94 %
Comment:
Propidium iodide (PI) is - like ethidium bromide (EtBr) - a DNA intercalator and was developed as an anti-Trypanosoma reagent, but never applied as such (1). Today, this fluorescence DNA dye is mainly applied in 'flow cytometry' (2, 3). At a concentration of 1 μg/ml, probably all DNA binding sites are saturated. PI will be excluded by living cells. Hence, only dead or dying cells will be stained. Propidium iodide can be excited by light with wavelenghts in the visible range and bleaches slowly, having clear advantages over DAPI (4).Stability: Depending on the reference, there are many recommendations of how to store solutions of Propidium iodide. According to ref. (4) store at +4°C (4) or at room temperature (3) or at -20°C (5) protecetd from light. The informations on the stability of solutions varies from 4-6 months (4) to unlimited (3). It seems that the buffer used is of great importance.Caution: Propidium iodide is hazardous, like all chemicals binding selectively to DNA. Propidium iodide (LD50 16 mg/kg, subcutaneous, mouse) is more toxic than ethidium bromide (LD50 110 mg/kg, subcutaneous, mouse).
Bibliography:
(1)Waring, M. (1975) Ethidium and Propidium in Antibiotics Vol. III, pages 141-165; (J.W. Corcoran & F.E. Hahn eds.) Springer-Verlag. (2)Pollack, A. & Ciancio, G. (1990) Methods Cell Biol. 33, 19-24Cell cycle phase-specific analysis of cell viability using Hoechst 33342 and Propidium iodide after Ethanol preservation. (3)Krishan, A. (1990) Methods Cell Biol. 33, 121-125Rapid DNA content analysis by the Propidium iodide-hypotonic citrate method. (4)Running, M.P. et al. (1995) Methods Cell Biol. 49, 217-229Confocal microscopy of the shoot apex. (5)Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology, page 14.7.10 (Suppl. 31) Greene Publishing & Wiley-Interscience, New York.
Hazard pictograms
  • GHS07 Hazard
  • GHS08 Hazard
WGK:
1
Storage:
2-8°C
protected from light
Signal Word:
Attention
GHS Symbols:
GHS07
GHS08
H Phrases:
H315
H319
H335
H341
P Phrases:
P261
P305+P351+P338
EINECS:
247-081-0
CS:
29339980
Download TDS file for complete specifications