DTT BioChemica

Assay (iodometr.): min. 99.5 %

Code: A1101
CAS: 3483-12-3
Molecular Formula: C₄H₁₀O₂S₂
Molar mass: 154.25 g/mol

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Packs sizes (6)

code	packaging size	price per unit	box price per unit
Code & packaging		Price per piece
code A1101,0005	packaging size 5 g	price per unit single $63,45	box price per unit $53,93x 6 units
code A1101,0025	packaging size 25 g	price per unit single $214,80	box price per unit $182,58x 6 units
code A1101,0100	packaging size 100 g	price per unit single $668,70	box price per unit
code A1101,1000	packaging size 1 kg	price per unit single Request a quote	box price per unit
code A1101,5000	packaging size 5 kg	price per unit single Request a quote	box price per unit
code A1101,9010	packaging size 10 kg	price per unit single Request a quote	box price per unit

Technical data

Solubility:: 1500 g/L (H₂O)
Physical Description:: Solid

Product Code:: A1101
Product Name:: DTT BioChemica
Headline Comment:: Attention: Product may form lumps when stored. Material is highly hygroskopic! Storage at 2°-8°C under inert gas! Open only under inert gas!
Specifications:: Assay (iodometr.): min. 99.5 %
pH (0.1 M; H₂O; 20°C): 4.0 - 6.0
Melting point: 40 - 44°C
DTT (oxidized): max. 0.5 %
Loss on drying: max. 0.5 %
A (1 cm/0.02 M in H₂O)
283 nm: max. 0.05
Comment:: Dithiothreitol (DTT) is, like β-mercaptoethanol, a reducing reagent for proteins and protects the cysteine residues against oxidation. It may substitute for β-mercaptoethanol in almost all experiments at three to four fold lower concentrations. DTT is less toxic, its odor is less intensive and it doesn't form mixed disulfides like β-mercaptoethanol. DTT is water-soluble and stock solutions are prepared at 1 M. Store the solution aliquoted at -20°C and protect from heat during the experiment. Do not choose a to low concentration for the experiment, because it is readily oxidized by air. The working concentration ranges from 0.1 to 1 mM, but the preparation of plant extracts ( 5 mM; ref. 4) or for the 'large scale in situ isolation' of proteins after fermentation (10 mM; ref. 5) require higher concentrations. For the complete reduction of disulfides, the concentration might be significantly higher (3).A more stable and odorless alternative to DTT is Tris(2-carboxy)ethylphosphine (A2233).
Bibliography:: (1)Cleland, W.W. (1964) Biochemistry 3, 480-482Dithiothreitol, a new protective reagent for SH groups. (2)Zahler, W.L. & Cleland, W.W. (1968) J. Biol. Chem. 243, 716-719A specific and sensitive assay for disulfides. (3)Jocelyn, P.C. (1987) Methods Enzymol. 143, 246-256Chemical reduction of disulfides. (4)Gegenheimer, P. (1990) Methods Enzymol. 182, 174-193Preparation of plant extracts. (5)Hart, R.A. et al. (1994) Bio/Technology 12, 1113-1117'Large scale in situ isolation' o periplasmatic IGF-I from E. coli.