Guanidine Thiocyanate for molecular biology

Assay (titr.): min. 99 %

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code A1107,0100	packaging size 100 g	price per unit single Request a quote	box price per unit
code A1107,0250	packaging size 250 g	Product discontinued. Alternative A1107,0100 (please check specifications).
code A1107,0500	packaging size 500 g	price per unit single $204,60	box price per unit
code A1107,1000	packaging size 1 kg	price per unit single $346,80	box price per unit
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Product Code:: A1107
Product Name:: Guanidine Thiocyanate for molecular biology
Specifications:: DNases/RNases/Proteases: not detectable
Assay (titr.): min. 99 %
pH (1 M): 4.7 - 7.0
Water (K.F.): max. 1.0 %
Ammonium: max. 0.1 %
Fe: max. 0.0005 %
A (1 cm/6 M in water HPLC grade)
280 nm: max. 0.6
300 nm: max. 0.1
Comment:: Reference (1) describes the original protocol for the guanidine thiocyanate (GuaSCN) / β-mercaptoethanol methode for the isolation of non-degraded RNA from RNase-rich tissues (e. g. pancreas). There are many variations from this method published (e. g. ref. 3, 4).As an alternative to the toxic formaldehyde, guanidine thiocyanate may be applied for Northern blotting (5). A final concentration of 20 mM is sufficient to denature RNA in the agarose gel (5).
Bibliography:: (1)Chirgwin, J.M. et al. (1979) Biochemistry 18, 5294-5299227Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. (2)MacDonald, R.J. et al. (1987) Methods Enzymol. 152, 219-227Isolation of RNA using guanidinium salts. (3)Lizardi, P.M. (1983) Methods Enzymol. 96, 24-38Methods of the preparation of messenger RNA. (4)Chomczynski, P. & Sacchi, N. (1987) Anal. Biochem. 162, 156-159Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. (5)Goda, S.K. & Minton, N.P. (1995) Nucleic Acids. Res. 23, 3357-3358A simple procedure for gel electrophoresis and Northern blotting of RNA.