Packs sizes (5)
code | packaging size | price per unit | box price per unit | |
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Code & packaging | Price per piece | |||
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code
A1107,0100
|
packaging size
100 g
|
price per unit
single
Request a quote
|
box price per unit
|
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code
A1107,0250
|
packaging size
250 g
|
Product discontinued. Alternative A1107,0100 (please check specifications).
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code
A1107,0500
|
packaging size
500 g
|
price per unit
single
$204,60
|
box price per unit
|
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code
A1107,1000
|
packaging size
1 kg
|
price per unit
single
$346,80
|
box price per unit
|
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code
A1107,9025
|
packaging size
25 kg
|
price per unit
single
Request a quote
|
box price per unit
|
Technical data
- Solubility:
- 1420 g/L (H2O)
- Physical Description:
- Solid
- Product Code:
- A1107
- Product Name:
- Guanidine Thiocyanate for molecular biology
- Specifications:
- DNases/RNases/Proteases: not detectable
Assay (titr.): min. 99 %
pH (1 M): 4.7 - 7.0
Water (K.F.): max. 1.0 %
Ammonium: max. 0.1 %
Fe: max. 0.0005 %
A (1 cm/6 M in water HPLC grade)
280 nm: max. 0.6
300 nm: max. 0.1
- Comment:
Reference (1) describes the original protocol for the guanidine thiocyanate (GuaSCN) / β-mercaptoethanol methode for the isolation of non-degraded RNA from RNase-rich tissues (e. g. pancreas). There are many variations from this method published (e. g. ref. 3, 4). As an alternative to the toxic formaldehyde, guanidine thiocyanate may be applied for Northern blotting (5). A final concentration of 20 mM is sufficient to denature RNA in the agarose gel (5).
- Bibliography:
(1) Chirgwin, J.M. et al. (1979) Biochemistry 18, 5294-5299227 Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. (2) MacDonald, R.J. et al. (1987) Methods Enzymol. 152, 219-227 Isolation of RNA using guanidinium salts. (3) Lizardi, P.M. (1983) Methods Enzymol. 96, 24-38 Methods of the preparation of messenger RNA. (4) Chomczynski, P. & Sacchi, N. (1987) Anal. Biochem. 162, 156-159 Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. (5) Goda, S.K. & Minton, N.P. (1995) Nucleic Acids. Res. 23, 3357-3358 A simple procedure for gel electrophoresis and Northern blotting of RNA.
- Hazard pictograms
-
- WGK:
- 2
- Storage:
- RT
- Signal Word:
- Attention
- GHS Symbols:
- GHS07
- H Phrases:
- EUH032
H302+H312+H332
H412
- P Phrases:
- P273
P302+P352
- EINECS:
- 209-812-1
- CS:
- 29252900
- Index Nr.:
- 615-004-00-3