Propidium Iodide BioChemica

Assay (HPLC): min. 94 %

Code: A2261
CAS: 25535-16-4
Molecular Formula: C₂₇H₃₄I₂N₄
Molar mass: 668.40 g/mol

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code	packaging size	price per unit	box price per unit
Code & packaging		Price per piece
code A2261,0025	packaging size 25 mg	price per unit single $88,95	box price per unit
code A2261,0100	packaging size 100 mg	price per unit single $290,85	box price per unit

Technical data

Physical Description:: Solid

Product Code:: A2261
Product Name:: Propidium Iodide BioChemica
Specifications:: Assay (HPLC): min. 94 %
Comment:: Propidium iodide (PI) is - like ethidium bromide (EtBr) - a DNA intercalator and was developed as an anti-Trypanosoma reagent, but never applied as such (1). Today, this fluorescence DNA dye is mainly applied in 'flow cytometry' (2, 3). At a concentration of 1 μg/ml, probably all DNA binding sites are saturated. PI will be excluded by living cells. Hence, only dead or dying cells will be stained. Propidium iodide can be excited by light with wavelenghts in the visible range and bleaches slowly, having clear advantages over DAPI (4).Stability: Depending on the reference, there are many recommendations of how to store solutions of Propidium iodide. According to ref. (4) store at +4°C (4) or at room temperature (3) or at -20°C (5) protecetd from light. The informations on the stability of solutions varies from 4-6 months (4) to unlimited (3). It seems that the buffer used is of great importance.Caution: Propidium iodide is hazardous, like all chemicals binding selectively to DNA. Propidium iodide (LD₅₀ 16 mg/kg, subcutaneous, mouse) is more toxic than ethidium bromide (LD₅₀ 110 mg/kg, subcutaneous, mouse).
Bibliography:: (1)Waring, M. (1975) Ethidium and Propidium in Antibiotics Vol. III, pages 141-165; (J.W. Corcoran & F.E. Hahn eds.) Springer-Verlag. (2)Pollack, A. & Ciancio, G. (1990) Methods Cell Biol. 33, 19-24Cell cycle phase-specific analysis of cell viability using Hoechst 33342 and Propidium iodide after Ethanol preservation. (3)Krishan, A. (1990) Methods Cell Biol. 33, 121-125Rapid DNA content analysis by the Propidium iodide-hypotonic citrate method. (4)Running, M.P. et al. (1995) Methods Cell Biol. 49, 217-229Confocal microscopy of the shoot apex. (5)Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology, page 14.7.10 (Suppl. 31) Greene Publishing & Wiley-Interscience, New York.