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Cacodylic Acid Sodium Salt 3-hydrate BioChemica

Assay (titr.): min. 98 %
Code
A2140
CAS
6131-99-3
Molecular Formula
C2H6AsNaO2 · 3H2O
Molar mass
214.03 g/mol
Solubility:
2000 g/L (H2O)
Physical Description:
Solid
Product Code:
A2140
Product Name:
Cacodylic Acid Sodium Salt 3-hydrate BioChemica
Specifications:
Assay (titr.): min. 98 %
Solubility (10 %; H2O): clear, colorless
Chloride: max. 0.002 %
Sulfate: max. 0.002 %
Cd: max. 0.005 %
Cu: max. 0.005 %
Fe: max. 0.005 %
Pb: max. 0.005 %
Zn: max. 0.005 %
Comment:
Sodium cacodylate is a widely used buffer for different assays\: (I) enzyme reaction buffer (e. g. ref. 1, 4)\: labeling of DNA at the 3' end with [α-32P]ddATP in 140 mM sodium cacodylate buffer (pH 7.2, ref. 4; pH 6.8 ref. 1) and terminal transferase; (II) reaction buffer for the chemical modification / cleavage, e. g. for the sequencing of DNA (50 mM, pH 8.0, ref. 5); (III) fixation of tissue, membranes and cells with glutaraldehyde or formaldehyde, respectively, in fixation buffer (e. g. ref. 2, 3). The working concentration ranges from 10 - 50 mM and a pH value of 7.2. Please note, that cacodylate inhibits the alkaline phosphatase in a concentration dependent manner in the cytochemical assay for this enzyme. As an alternative, the authors recommend the use of a NaOH-Pipes buffer (2)
Bibliography:
(1)Okayma, H. & Berg, P. (1982) Mol. Cell. Biol. 2, 161-170High-efficiency cloning of full-length cDNA. (2)Yamamoto, K. & Ogawa, K. (1983) Histochemistry 77, 339-351Effects of NaOH-PIPES buffer used in aldehyde fixative on alkaline Phosphatase activity in rat hepatocytes. (3)Hardham, A.R. (1985) J. Histochem. Cytochem. 33, 110-118Studies on the cell surface of zoospores and cysts of the fungus Phytophthora cinnamomi\: The influence of fixation on patterns of Lectin binding. (4)Cobianchi, F. & Wilson, S.H. (1987) Methods Enzymol. 152, 94-110Enzymes for modifying and labeling DNA and RNA. (5)Ambrose, B.J.B. & Pless, R.C. (1987) Methods Enzymol. 152, 522-538DNA Sequencing\: Chemical methods. (6)McCarthy, J.G. et al. (1990) Biochemistry 29, 6071-6081Chemical reactivity of potassium permanganate and diethyl pyrocarbonate with B DNA\: Specific reactivity with short A-tracts.
Hazard pictograms
  • GHS06 Hazard
  • GHS09 Hazard
UN:
1688
Class/PG:
6.1/II
ADR:
6.1/II
IMDG:
6.1/II
IATA:
6.1/II
WGK:
3
Storage:
RT
Signal Word:
Danger
GHS Symbols:
GHS06
GHS09
H Phrases:
H301+H331
H410
P Phrases:
P261
P301+P310
P321
P330
P405
P501
EINECS:
204-708-2
CS:
29319000
Index Nr.:
033-002-00-5
Download TDS file for complete specifications