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DTE BioChemica

Assay (iodometr.): min. 99 %
Code
A1102
CAS
6892-68-8
Molecular Formula
C4H10O2S2
Molar mass
154.25 g/mol
Physical Description:
Solid
Product Code:
A1102
Product Name:
DTE BioChemica
Specifications:
Assay (iodometr.): min. 99 %
Solubility (5 %; H2O): clear, colorless
pH (0.1 M; H2O; 20°C): 4.0 - 6.0
Comment:
Dithioerythritol (DTE) is an isomere of dithiothreitol (DTT). In general, DTE and DTT are exchangeable. DTE has pK values of 9.0 and 9.9 and DTT of 8.3 and 9.5, respectively. Both reagents function in the ionized state only. Therefore the reducing activity is highest in the pH range of 7.0 - 9.5. At lower pH values DTT is more active than DTE. The reducing activity is stopped by acidifying the pH (e. g. acetic acid at pH 3; ref. 3).Proteins may oxidize during gel electrophoresis. This results in diffuse bands (decreased resolution) and a non-reproducible mobility (wrong estimation of molecular size). Reduction of the protein by DTE (0.25 M stock solution in water; 5 mM final concentration) and alkylation by iodoacetamide (0.25 M stock solution; 1 \: 20 diluted in the final concentration) solves the problem (2).
Bibliography:
(1)Cleland, W.W. (1964) Biochemistry 3, 480-482Dithiothreitol, a new protective reagent for SH groups. (2)Lane, L.C. (1978) Anal. Biochem. 86, 655-664A simple method for stabilizing protein-sulfhydryl groups during SDS-gel electrophoresis. (3)Jocelyn, P.C. (1987) Methods Enzymol. 143, 246-256Chemical reduction of disulfides.
Hazard pictograms
  • GHS07 Hazard
WGK:
1
Storage:
2-8°C
Signal Word:
Attention
GHS Symbols:
GHS07
H Phrases:
H315
H319
H335
P Phrases:
P261
P280
P304+P340
P305+P351+P338
P405
P501
EINECS:
229-998-8
CS:
29309098
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