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Guanidine Thiocyanate for molecular biology

Assay (titr.): min. 99 %
Code
A1107
CAS
593-84-0
Molecular Formula
CH6N3SCN
Molar mass
118.16 g/mol

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Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A1107,0100
code
A1107,0100
packaging size
100 g
price per unit
single $63,00
box price per unit
A1107,0250
code
A1107,0250
packaging size
250 g
price per unit
single $109,65
box price per unit
A1107,0500
code
A1107,0500
packaging size
500 g
price per unit
single $194,85
box price per unit
A1107,1000
code
A1107,1000
packaging size
1 kg
price per unit
single $338,25
box price per unit
A1107,9025
code
A1107,9025
packaging size
25 kg
price per unit box price per unit
Solubility:
1420 g/L (H2O)
Physical Description:
Solid
Product Code:
A1107
Product Name:
Guanidine Thiocyanate for molecular biology
Specifications:
DNases/RNases/Proteases: not detectable
Assay (titr.): min. 99 %
pH (1 M): 4.7 - 7.0
Water (K.F.): max. 1.0 %
Ammonium: max. 0.1 %
Fe: max. 0.0005 %
A (1 cm/6 M in water HPLC grade)
280 nm: max. 0.6
300 nm: max. 0.1
Comment:
Reference (1) describes the original protocol for the guanidine thiocyanate (GuaSCN) / β-mercaptoethanol methode for the isolation of non-degraded RNA from RNase-rich tissues (e. g. pancreas). There are many variations from this method published (e. g. ref. 3, 4).As an alternative to the toxic formaldehyde, guanidine thiocyanate may be applied for Northern blotting (5). A final concentration of 20 mM is sufficient to denature RNA in the agarose gel (5).
Bibliography:
(1)Chirgwin, J.M. et al. (1979) Biochemistry 18, 5294-5299227Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. (2)MacDonald, R.J. et al. (1987) Methods Enzymol. 152, 219-227Isolation of RNA using guanidinium salts. (3)Lizardi, P.M. (1983) Methods Enzymol. 96, 24-38Methods of the preparation of messenger RNA. (4)Chomczynski, P. & Sacchi, N. (1987) Anal. Biochem. 162, 156-159Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. (5)Goda, S.K. & Minton, N.P. (1995) Nucleic Acids. Res. 23, 3357-3358A simple procedure for gel electrophoresis and Northern blotting of RNA.
Hazard pictograms
  • GHS07 Hazard
WGK:
2
Storage:
RT
Signal Word:
Attention
GHS Symbols:
GHS07
H Phrases:
EUH032
H302+H312+H332
H412
P Phrases:
P273
P302+P352
EINECS:
209-812-1
CS:
29252900
Index Nr.:
615-004-00-3
Download TDS file for complete specifications