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Spermine BioChemica

Assay (titr.): min. 98 %
Code
A0672
CAS
71-44-3
Molecular Formula
C10H26N4
Molar mass
202.34 g/mol
Physical Description:
Solid
Product Code:
A0672
Product Name:
Spermine BioChemica
Specifications:
Assay (titr.): min. 98 %
pH (1 M; H2O; 20°C): 11.0 - 13.0
Spermidine: max. 0.5 %
Water: max. 1.0 %
Carbonate: max. 0.5 %
Comment:
Spermine belongs to the Polyamines. When precipitating selectivly DNA with the help of Spermine most of the proteins and triphosphates will not coprecipitate. The precipitation with spermidine is applied after restriction digests, the separation of DNA fragments and triphosphates and the purification of DNA fragments after PAGE. Stock solutions are prepared with a concentration of 100mM (pH 6.8, ref. 2) and stored at \+4°C (short-term) or at -20°C (long-term). The working concentration is approx 0.1 mM, if the DNA is dissolved in TE.
Bibliography:
(1)Blumenthal, A.B. et al. (1979) J. Cell Biol. 81, 255-259Rapid isolation of Metaphase chromosomes containing high molecular weight DNA. (2)Hoopes, B. & McClure, W. (1981) Nucleic Acids Res. 9, 5493-5504Studies on the selectivity of DNA precipitation by spermine. (3)Tabor, C.W. & Tabor, H. (1984) Ann. Rev. Biochem. 53, 749-790Review article\: Polyamines. (4)Cram, L.S. et al. (1990) Methods Cell Biol. 33, 377-382Polyamine buffer for bivariate human flow cytogenetic analysis and sorting.
Hazard pictograms
  • GHS05 Hazard
UN:
3259
Class/PG:
8/II
ADR:
8/II
IMDG:
8/II
IATA:
8/II
WGK:
1
Storage:
2-8°C
Signal Word:
Danger
GHS Symbols:
GHS05
H Phrases:
H314
P Phrases:
P280
P305+P351+P338
P313
EINECS:
200-754-2
CS:
29212900
Download TDS file for complete specifications