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Rhodamine B (C.I. 45170)

Assay (photometr.): min. 90 %
Code
A3930
CAS
81-88-9
Molecular Formula
C28H31ClN2O3
Molar mass
479.02 g/mol

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Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A3930,0100
code
A3930,0100
packaging size
100 g
price per unit
single 101,70€
box price per unit
molecule for: Rhodamine B (C.I. 45170)
Solubility:
34 g/L (H2O)
Physical Description:
Solid
Product Code:
A3930
Product Name:
Rhodamine B (C.I. 45170)
Specifications:
Assay (photometr.): min. 90 %
λmax. (50 % EtOH): 550 - 552 nm
E 1 %/1 cm, λmax.: 2115 - 2350
pH (0.5 %; H2O; 20°C): approx. 2.0
Loss on drying: max. 5 %
UV/VIS spectrum: passes test
Hazard pictograms
  • GHS05 Hazard
  • GHS07 Hazard
WGK:
2
Storage:
RT
Signal Word:
Danger
GHS Symbols:
GHS05
GHS07
H Phrases:
H302
H318
H412
P Phrases:
P280
P301+P312
P305+P351+P338
P310
P330
P501
EINECS:
201-383-9
CS:
32041300
Download TDS file for complete specifications

Comments

The mixed-dye staining method of proteins in polyacrylamide gels with Eriochrome black T in combination with Rhodamine B can detect as little as 10 ng of BSA within one hour (1) and is more sensitive than Coomassie®-staining. The optimum dye concentration of Eriochrome Black T was determined to be 0.01 %. Protein bands were scanned at 560 nm by densitometer. Rhodamine B was employed at the same concentration (0.01 %). Staining solution was prepared by mixing stock solutions of Eriochrome Black T (0.02 % (w/v)) and Rhodamine B (0.02 % (w/v)) in 40 % methanol/7 % acetic acid in a ratio of 1 : 1 (v/v) just prior to use. Besides sensitivity, another advantage of the mixed-dye staining method over Coomassie® is the stability of the staining pattern after drying (60°C, 30 min; ref. 1).

Literature

(1) Jung, D.-W. et al. (1998) Anal. Biochem. 263, 118-120. Detection of Proteins in Polyacylamide Gels Using Eriochrome Black T and Rhodamine B.