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3,3',5,5'-Tetramethylbenzidine (TMB) BioChemica

Assay (titr.): min. 98 %
Code
A3840
CAS
54827-17-7
Molecular Formula
C16H20N2
Molar mass
240.35 g/mol

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Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A3840,0001
code
A3840,0001
packaging size
1 g
price per unit
single $42,75
box price per unit
A3840,0005
code
A3840,0005
packaging size
5 g
price per unit
single $145,20
box price per unit
A3840,0025
code
A3840,0025
packaging size
25 g
price per unit
single $484,20
box price per unit
A3840,0250
code
A3840,0250
packaging size
250 g
price per unit
single $3879,45
box price per unit
Solubility:
insoluble (H2O)
Physical Description:
Solid
Product Code:
A3840
Product Name:
3,3',5,5'-Tetramethylbenzidine (TMB) BioChemica
Specifications:
Assay (titr.): min. 98 %
Residue on ignition: max. 0.2 %
Loss on drying: max. 0.5 %
WGK:
2
Storage:
2 - 8°C
under argon
EINECS:
259-364-6
CS:
29215990
Download TDS file for complete specifications

Comments

Tetramethylbenzidine (TMB) is used as a chromogenic substrate for the detection of horseradish peroxidase, especially applied in Enzyme Immunoassays (e. g. ELISA). It is more sensitive than o-phenylene diamine or ABTS and more stable and less toxic than diaminobenzidine. TMB has been classified as nontoxic. The color development is generally completed after 10 - 30 minutes at room temperature. The blue color turns to yellow when the reaction is stopped by the addition of 1 M sulfuric acid and the color development is measured at 450 nm.Solubility: TMB is soluble in DMSO, acetone, chloroform, toluene (100 mg/ml) or ethanol 96 % (~30 mg/ml). It is insoluble in water unless TMB forms the dihydrochloride. Stock solutions can be prepared e.g. as 0.01 M solutions (0.24 g/100 ml) up to 1.5 % (1.5 g/100 ml). Solutions are stored at 2-8°C.

Literature

(1)Volpe, G. et al. (1998) Analyst 123, 1303-1307TMB as electrochemical substrate for horseradish peroxidase based immunoassays. A comparative study. (2)Schütz, A.J. et al. (1997) SPIE Proc. 3105, 332-340Stabilization of Horseradish Peroxidase (HRP) for the Use in Immunochemical Sensors.