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Acrylamide solution (40 %) - Mix 19 : 1 for molecular biology

Code
A3658

Recommended prices only. For prices and orders please contact your local distributor.
Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A3658,0500
code
A3658,0500
packaging size
500 ml
Product active until stock lasts.
A3658,1000
code
A3658,1000
packaging size
1 L
price per unit
single $205,35
box price per unit
Physical Description:
Liquid
Product Code:
A3658
Product Name:
Acrylamide solution (40 %) - Mix 19 : 1 for molecular biology
Short Description:
additional product description: Aqueous solutions
Specifications:
DNases/RNases/Proteases: not detectable
Acrylic Acid (titr.): max. 0.001 %
Composition:
Acrylamide: 380.0 g/L
Bisacrylamide: 20.0 g/L
Hazard pictograms
  • GHS06 Hazard
  • GHS08 Hazard
UN:
3426
Class/PG:
6.1/III
ADR:
6.1/III
IMDG:
6.1/III
IATA:
6.1/III
WGK:
3
Storage:
RT
Signal Word:
Danger
GHS Symbols:
GHS06
GHS08
H Phrases:
H301
H312+H332
H315
H317
H319
H340
H350
H361f
H372
P Phrases:
P280
P301+P310
P305+P351+P338
P321
P330
P362+P364
P405
P501
CS:
38221900
Index Nr.:
616-003-00-0
Download TDS file for complete specifications

Comments

For the separation of oligonucleotides with a small number of bases, acrylamide gels with a small pore size are used. The pore size is determined by the total concentration of acrylamide and the ratio of acrylamide : bisacrylamide. For the sequencing of nucleic acids a ratio of acrylamide : bisacrylamide of 19 : 1 is used. Caution: Acrylamide is a potent neurotoxin and absorbed through the skin. The effects of acrylamide are cumulative. Although polyacrylamide is suspected to be nontoxic, it should be handled with great care because of the possibility that it might contain small quantities of unpolymerized acrylamide.

Literature

(1) Peacock, A.C. & Dingman, C.W. (1967) Biochemistry 7, 668-674. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. (2) Ambrose, B.J.B. & Pless, R.C. (1987) Methods Enzymol. 152, 522-538. DNA sequencing: Chemical methods. (3) Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. (4) Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology. Greene Publishing & Wiley-Interscience, New York.