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Aprotinin BioChemica

Assay (protein): min. 90 %
Code
A2132
CAS
9087-70-1
Molecular Formula
C284H432N84O79S7
Molar mass
6511.52 g/mol

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Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A2132,0010
code
A2132,0010
packaging size
10 mg
price per unit
single $49,35
box price per unit
$41,95x 6 units
A2132,0025
code
A2132,0025
packaging size
25 mg
price per unit
single $97,95
box price per unit
A2132,0100
code
A2132,0100
packaging size
100 mg
price per unit
single $344,70
box price per unit
Physical Description:
Solid
Product Code:
A2132
Product Name:
Aprotinin BioChemica
Headline Comment:
® registered trademark of Bayer AG
Specifications:
Assay (protein): min. 90 %
Activity (KIU/mg): min. 5500
pH (1 %; H2O): 5.0 - 7.5
Water (K.F.): max. 6 %
Comment:
Aprotinin was isolated independently from two laboratories and originally named Bovine Pancreatic Trypsin Inhibitor (BPTI) and Trypsin-kallikrein Inhibitor (TKI). It is a strongly basic protein (58 amino acids; ref. 1, 2). Aprotinin inhibits trypsin, chymotrypsin, kallikrein from different sources and plasmin. Its recommended working concentration is 2 - 10 μg/ml (approx. 1 μM). The working concentration given in ref. 3 (500 KIU/ml; approx. 10 μM) seems to be very high.Stability\: Aprotinin may be stored indefinite at \+4°C in lyophilized form. Dissolved in saline or buffer solutions (e. g. 10 mg/ml) at pH 5 - 8, it may be stored aliquoted at \+4°C at least for one month or frozen at -20°C for several years. Thawed aliquots of the stock solutions should not be frozen again, because repeated freeze-thawing will lead to aggregation of the polypeptide aprotinin. Solutions with a pH value below 4 or above 9 should be used immediately. Protect from direct sunlight, UV light and reducing agents. It is stable in water, 70 % methanol, 70 % ethanol or 50 % acetone, too (2, 3).Unit-Definition: One Trypsin Inhibitor Unit (TIU) will decrease the activity of 2 trypsin units by 50 %, where one trypsin unit will hydrolyze 1.0 μmole Nα-benzoyl-DL-arginine p-nitroanilide (BAPNA) per minute at pH 7.8 and 25°C. 1 TIU (Trypsin Inhibitor Unit) corresponds to approx. 1300 KIU (Kallikrein Inhibitor Units) and 1 TIU corresponds to one Ph. Eur. Unit.
Bibliography:
(1)Kassell, B. & Laskowski, Sr., M. (1965) Biochem. Biophys. Res. Com. 20, 463-468.The basic Trypsin inhibitor of bovine pancreas\: V. The disulfide linkages. (2)Kassell, B. (1970) Methods Enzymol. 19, 844-852Bovine Trypsin-Kallikrein inhibitor (Kunitz Inhibitor, Basic Pancreatic Trypsin Inhibitor, Polyvalent Inhibitor from Bovine Organs). (3)Fritz, H. & Wunderer, G. (1983) Drug Res. 33, 479-494Biochemistry and application of Aprotinin, the Kallikrein inhibitor from bovine organs.
WGK:
1
Storage:
2-8°C
Origin:
from bovine lung
CS:
35040090
Download TDS file for complete specifications