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Cetyltrimethylammonium Bromide (CTAB) BioChemica

Assay (titr.): min. 99 %
Code
A0805
CAS
57-09-0
Molecular Formula
C19H42BrN
Molar mass
364.46 g/mol

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Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A0805,0100
code
A0805,0100
packaging size
100 g
price per unit
single $42,45
box price per unit
A0805,0500
code
A0805,0500
packaging size
500 g
price per unit
single $159,00
box price per unit
molecule for: Cetyltrimethylammonium Bromide (CTAB) BioChemica
Solubility:
3 g/L (H2O)
Physical Description:
Solid
Product Code:
A0805
Product Name:
Cetyltrimethylammonium Bromide (CTAB) BioChemica
Specifications:
Assay (titr.): min. 99 %
Heavy metals (as Pb): max. 0.001 %
Water (K.F.): max. 1 %
Fe: max. 0.001 %
Hazard pictograms
  • GHS05 Hazard
  • GHS07 Hazard
  • GHS09 Hazard
UN:
3077
Class/PG:
9/III
ADR:
9/III
IMDG:
9/III
IATA:
9/III
WGK:
3
Storage:
RT
Signal Word:
Danger
GHS Symbols:
GHS05
GHS07
GHS09
H Phrases:
H302
H315
H318
H335
H400
P Phrases:
P261
P305+P351+P338
P310
P321
P330
P362+P364
P405
P501a
EINECS:
200-311-3
CS:
29239000
Download TDS file for complete specifications

Comments

Cethyltrimethylammonium bromide (CTAB) is a cationic detergent. In polyacrylamide gel electrophoresis it is used for the determination of the molecular weight of proteins, which show an unusual migration behavior in the SDS-PAGE (e. g. strongly charged proteins or subunits of membrane proteins). See reference 1 for details of this method.Another important application of CTAB is the precipitation of high molecular weight DNA, especially from plant material (genomic DNA; ref. 3, 5). CTAB forms insoluble complexes with the nucleic acids (RNA, too!; ref. 6), if the NaCl-concentration is decreased to approx. 0.5 M. The tissue or cells are homogenized in CTAB-containing buffers. The effective concentration is 1 - 2 %. Keep the temperature above 15°C, otherwise CTAB will precipitate. Disturbing phenolic substances, which are very prominent in plant material, will not coprecipitated by this method. Nevertheless, in case that they are still disturbing, they can be removed with polyvinylpyrrolidone (K30, ref. 5; K40, ref. 6). The complexes of nucleic acids and CTAB can be dissolved at high salt concentrations only. The detergent is removed by ethanol precipitation and washing of the precipitate with 80 % ethanol, since it is more soluble in ethanol.Stability\: Solutions of CTAB are stable for several years at room temperature (5).

Literature

(1)Eley, M.H. et al. (1979) Anal. Biochem. 92, 411-419CTAB-PAGE\: Estimation of protein subunit molecular weights using cationic detergents. (2)Hansen, S.H. et al. (1981) Chromatographia 13, 453-460HPLC on dynamicaly modified silica\: II. Modification of various silica packings with CTAB. (3)Murray, M.G. & Thompson, W.F. (1980) Nucleic Acids Res. 8, 4321-4325Rapid isolation of high molecular weight plant DNA. (4)Shahjahan, R.M. et al. (1995) BioTechniques 19, 332-334Lower incubation temperature increases the yield of genomic DNA from insect tissue. (5)Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1999) Current Protocols in Molecular Biology. Page 2.3.3-2.3.7, 2.4.2 (Suppl. 27) Greene Publishing & Wiley-Interscience, New York. (6)White, E.J. et al. (2008) Biotechnol. J. 3, 1424-1428Modified CTAB method improves robustness and versatility: The benchmark for plant RNA extraction.