HEPES for buffer solutions

Assay (titr.): min. 99.5 %

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code	packaging size	price per unit	box price per unit
Code & packaging		Price per piece
code A1069,0100	packaging size 100 g	price per unit single $43,65	box price per unit
code A1069,0250	packaging size 250 g	price per unit single $102,30	box price per unit
code A1069,0500	packaging size 500 g	price per unit single $159,90	box price per unit
code A1069,1000	packaging size 1 kg	price per unit single $262,95	box price per unit $223,51x 6 units
code A1069,5000	packaging size 5 kg	price per unit single $1135,20	box price per unit
code A1069,9020	packaging size 20 kg	price per unit single Request a quote	box price per unit
code A1069,9025BW	packaging size 25 kg	price per unit single Request a quote	box price per unit

Product Code:: A1069
Product Name:: HEPES for buffer solutions
Specifications:: Assay (titr.): min. 99.5 %
pH (1 %; H₂O): 4.7 - 6.0
Heavy metals (as Pb): max. 0.001 %
Water: max. 0.5 %
Chloride: max. 0.05 %
Sulfate: max. 0.05 %
Comment:: Hepes is a widely used buffer in biological studies. There is only one restriction in the use of this buffer, because it interferes with the Folin protein assay. In cell culture media, it is employed as a substitute for the bicarbonate buffer at a concentration of 25 mM or as a supplement to the bicarbonate buffer (concentration 10 - 15 mM).The addition of 20 mM HEPES to TBE buffer improves the migration behaviour of certain samples in the SSCP analysis (single-strand conformation polymorphism), with a higher resolution especially if small differences in the sequences between certain bands are wanted (5).
Bibliography:: (1)Good, N.E. et al. (1966) Biochemistry 5, 467-477Hydrogen ion buffers for biological research. (2)Shipman, C. (1969) Proc. Soc. Exp. Biol. Med. 130, 305-310Evaluation of Hepes as a tissue culture buffer. (3)Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68Hydrogen ion buffers. (4)Ferguson, W.J. et al. (1980) Anal. Biochem. 104, 300-310Hydrogen ion buffers for biological research. (5)Liu, Q. & Sommer, S.S. (1998) Biotechniques 25, 50-56The SSCP-Phenomenon\: Addition of HEPES influences the electrophoretical mobility.