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AEBSF Hydrochloride BioChemica

Assay (titr., calc. on dried substance): min. 98 %
Code
A1421
CAS
30827-99-7
Molecular Formula
C8H10FNO2S · HCl
Molar mass
239.69 g/mol

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Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A1421,0100
code
A1421,0100
packaging size
100 mg
price per unit
single $51,60
box price per unit
A1421,0250
code
A1421,0250
packaging size
250 mg
price per unit
single $113,70
box price per unit
A1421,0500
code
A1421,0500
packaging size
500 mg
price per unit
single $182,40
box price per unit
A1421,0001
code
A1421,0001
packaging size
1 g
price per unit
single $304,80
box price per unit
$259,08x 6 units
A1421,0005
code
A1421,0005
packaging size
5 g
price per unit box price per unit
A1421,9025
code
A1421,9025
packaging size
25 g
price per unit box price per unit
Solubility:
50 g/L (H2O)
Physical Description:
Solid
Product Code:
A1421
Product Name:
AEBSF Hydrochloride BioChemica
Specifications:
Assay (titr., calc. on dried substance): min. 98 %
Assay (HPLC): min. 98 %
Loss on drying: max. 1.0 %
Comment:
AEBSF irreversibly inactivates thrombin and other serine proteases (e. g. chymotrypsin, kallikrein, plasmin, proteinase K, trypsin) by sulfonylation of a functional group in the active center of the enzyme (1, 2). AEBSF (LD50 oral, mouse 2.8 g/kg) is a substantially less toxic substitute for PMSF and DFP. It is highly water-soluble and its solutions have an acidic pH. At this pH, stability is high. AEBSF is inactivated at 37°C by 50 % after 5 hours. We recommend storage of the stock solution (e. g. 20 mM or 100 mM in water or buffer) at -20°C (stable for up to 2 months; at \+4°C approx. 1 week; ref. 6). It should be added to the assay buffers just before use at a final concentration of 0.1 - 2 mM (e. g. ref. 3-5).
Bibliography:
(1)Walsmann, P. et al. (1972) Acta biol. med. germ. 28, 577-585Inactivation of Trypsin and Thrombin through 4-Amidinobenzene sulfonyl fluoride and 4-(2-Aminoethyl)-benzene sulfonyl fluoride. (2)Markwardt, F. et al. (1973) Thrombosis Res. 2, 343-348Synthetic low molecular weight inhibitors of serum kallikrein. (3)Murphy,. B.J. et al. (1993) J. Biol. Chem. 268, 27355-27362Identification of the sites of selective phosphorylation and dephosphorylation of the rat brain Na+ channel a subunit by cAMP-dependent protein kinase and phosphoprotein phosphatases. (4)Nathan, D.F. & Lindquist. S. (1995) Mol. Cell. Biol. 15, 3917-3925Mutational analysis of HSP90 function\: Interactions with a steroid receptor and a protein kinase. (5)Taylor, J.A. et al. (1995) Mol. Cell. Biol. 15, 4149-4157Activation of the high-affinity immunoglobulin E receptor FceRI in RBL-2H3 cells is inhibited by syk SH2 domains. (6)Taylor, J.A. et al. (1995) Immunology 86, 629-635Serine protease inhibitors block the priming of monocytes for enhanced superoxide release.
Hazard pictograms
  • GHS05 Hazard
WGK:
1
Storage:
2-8°C
Signal Word:
Danger
GHS Symbols:
GHS05
H Phrases:
H314
P Phrases:
P280
P305+P351+P338
P310
CS:
29214900
Download TDS file for complete specifications