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DTT BioChemica

Assay (iodometr.): min. 99.5 %
Code
A1101
CAS
3483-12-3
Molecular Formula
C4H10O2S2
Molar mass
154.25 g/mol

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Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A1101,0005
code
A1101,0005
packaging size
5 g
price per unit
single $58,95
box price per unit
$50,11x 6 units
A1101,0025
code
A1101,0025
packaging size
25 g
price per unit
single $207,60
box price per unit
$176,46x 6 units
A1101,0100
code
A1101,0100
packaging size
100 g
price per unit
single $646,80
box price per unit
A1101,1000
code
A1101,1000
packaging size
1 kg
price per unit box price per unit
A1101,5000
code
A1101,5000
packaging size
5 kg
price per unit box price per unit
A1101,9010
code
A1101,9010
packaging size
10 kg
price per unit box price per unit
Solubility:
1500 g/L (H2O)
Physical Description:
Solid
Product Code:
A1101
Product Name:
DTT BioChemica
Headline Comment:
Attention: Product may form lumps when stored. Material is highly hygroskopic! Storage at 2°-8°C under inert gas! Open only under inert gas!
Specifications:
Assay (iodometr.): min. 99.5 %
pH (0.1 M; H2O; 20°C): 4.0 - 6.0
Melting point: 40 - 44°C
DTT (oxidized): max. 0.5 %
Loss on drying: max. 0.5 %
A (1 cm/0.02 M in H2O)
283 nm: max. 0.05
Comment:
Dithiothreitol (DTT) is, like β-mercaptoethanol, a reducing reagent for proteins and protects the cysteine residues against oxidation. It may substitute for β-mercaptoethanol in almost all experiments at three to four fold lower concentrations. DTT is less toxic, its odor is less intensive and it doesn't form mixed disulfides like β-mercaptoethanol. DTT is water-soluble and stock solutions are prepared at 1 M. Store the solution aliquoted at -20°C and protect from heat during the experiment. Do not choose a to low concentration for the experiment, because it is readily oxidized by air. The working concentration ranges from 0.1 to 1 mM, but the preparation of plant extracts ( 5 mM; ref. 4) or for the 'large scale in situ isolation' of proteins after fermentation (10 mM; ref. 5) require higher concentrations. For the complete reduction of disulfides, the concentration might be significantly higher (3).A more stable and odorless alternative to DTT is Tris(2-carboxy)ethylphosphine (A2233).
Bibliography:
(1)Cleland, W.W. (1964) Biochemistry 3, 480-482Dithiothreitol, a new protective reagent for SH groups. (2)Zahler, W.L. & Cleland, W.W. (1968) J. Biol. Chem. 243, 716-719A specific and sensitive assay for disulfides. (3)Jocelyn, P.C. (1987) Methods Enzymol. 143, 246-256Chemical reduction of disulfides. (4)Gegenheimer, P. (1990) Methods Enzymol. 182, 174-193Preparation of plant extracts. (5)Hart, R.A. et al. (1994) Bio/Technology 12, 1113-1117'Large scale in situ isolation' o periplasmatic IGF-I from E. coli.
Hazard pictograms
  • GHS07 Hazard
WGK:
1
Storage:
2 - 8°C
under inert gas
Shipment:
wet ice in Germany, dry ice to abroad
Signal Word:
Attention
GHS Symbols:
GHS07
H Phrases:
H302
H315
H319
P Phrases:
P302+P350
P305+P351+P338
EINECS:
222-468-7
CS:
29309098
Download TDS file for complete specifications