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Ficoll® 400 BioChemica

Code
A2252
CAS
26873-85-8
Molar mass
approx. 400000 g/mol
Physical Description:
Solid
Product Code:
A2252
Product Name:
Ficoll® 400 BioChemica
Short Description:
additional product description: non-ionic, synthetic polymere of sucrose
Headline Comment:
® registered trademark Pharmacia Biotech
Specifications:
Solubility (10 %; H2O): clear, colorless
Heavy metals (as Pb): max. 0.001 %
Loss on drying: max. 3 %
Comment:
Ficoll® 400 is a component of Denhardt's solution (see A2248 and ref. 1, 2 [Page 9.47-9.55]) and several gel loading buffers (2 [Page 6.12]). The addition of Ficoll® 400, sucrose or glycerol increase the density of the loading buffers and facilitate the loading of the sample onto the gel.Composition of loading buffers\: I. 0.25 % Bromophenol blue, 0.25 % xylene cyanol FF, 15 % Ficoll® 400 in water (store at room temperature)(according to ref. 2) II. alkaline loading buffer 300 mM NaOH, 6 mM EDTA, 18 % Ficoll® 400, 0.15 % Bromocresol green, 0.25 % xylene cyanol FF in water (store at \+4°C)Have a look at our 9 different loading buffers.
Bibliography:
(1)Denhardt, D.T. (1966) Biochem. Biophys. Res. Com. 23, 641-646A membrane-filter technique for the detection of complementary DNA. (2)Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning\: A Laboratory Manual, 2nd Edition; p. 9.47-9.55, 6.12. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
WGK:
1
Storage:
RT
EINECS:
200-334-9
CS:
39072020
Download TDS file for complete specifications