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PMSF BioChemica

Assay (GC): min. 99 %
Code
A0999
CAS
329-98-6
Molecular Formula
C7H7FO2S
Molar mass
174.19 g/mol

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Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A0999,0005
code
A0999,0005
packaging size
5 g
price per unit
single $41,70
box price per unit
$35,45x 6 units
A0999,0025
code
A0999,0025
packaging size
25 g
price per unit
single $148,05
box price per unit
A0999,0100
code
A0999,0100
packaging size
100 g
price per unit
single $412,20
box price per unit
A0999,1000
code
A0999,1000
packaging size
1 kg
price per unit box price per unit
A0999,9025
code
A0999,9025
packaging size
25 kg
price per unit box price per unit
Physical Description:
Solid
Product Code:
A0999
Product Name:
PMSF BioChemica
Specifications:
Assay (GC): min. 99 %
Solubility (10 %, EtOH): clear
Heavy metals (as Pb): max. 0.001 %
Sulfated ash: max. 0.1 %
Water (K.F.): max. 0.5 %
Comment:
PMSF is an irreversible inhibitor of serin-proteases like trypsin, chymotrypsin and cysteine proteases. The inhibition of cysteine proteases can be reverted by reducing thioles. It is frequently used for the preparation of proteins from cell lysates. PMSF is limited soluble in water. Stock solutions are prepared in organic solvents (e. g. 10 mg/ml in 10 % Isopropanol). The effective concentration of PMSF is 0.1 - 1 mM. The LD50 (i.p.) for the mouse is 200 mg/kg.Stability\: In aqueous solutions PMSF is inactivated very fast, especially at raising pH and temperature. Therefore, it has to be added fresh during each working step. At pH 7.0, 7.5 and 8.0 at 25°C the half life is 110, 55 and 35 minutes respectively. Stock solutions (Isopropanol) are stable for about 9 months at \+4°C (4). Stock solutions of PMSF (10 - 100 mM; 1.74 mg/ml - 17.4 mg/ml, respectively) are prepared with anhydrous Ethanol, Methanol or Isopropanol and stored at -20°C. PMSF may crystallize in Isopropanol at -20°C.Caution\: PMSF is extremely toxic to mucous membranes of the lung, eyes and skin. Any contact by inhalation, swallowing or contact with skin must be avoided. After contact with skin wash immediately with plenty of water. We recommend to use AEBSF instead of PMSF, which is as effective but substantially less toxic.
Bibliography:
(1)Fahrney, D.E. & Gold, A.M. (1963) J. Am. Chem. Soc. 85, 996-1000Sulfonyl fluorides as inhibitors of esterases. I. Rates of reaction with acetylcholinesterase, α-Chymotrypsin and trypsin. (2)Gold, A.M. & Fahrney, D.E. (1964) Biochemistry 3, 783-791Sulfonyl fluorides as inhibitors of esterases. II Formation and reactions of phenylmethanesulfonyl α-Chymotrypsin. (3)Prouty, W.F. & Goldberg, A.L. (1972) J. Biol. Chem. 247, 3341-3352Effects of protease inhibitors on protein breakdown in E. coli. (4)James, G.T. (1978) Anal. Biochem. 86, 574-579Inactivation of the protease inhibitor Phenylmethylsulfonyl fluoride in buffers.
Hazard pictograms
  • GHS05 Hazard
  • GHS06 Hazard
UN:
2923
Class/PG:
8(6.1)/II
ADR:
8(6.1)/II
IMDG:
8(6.1)/II
IATA:
8(6.1)/II
WGK:
1
Storage:
RT
Signal Word:
Danger
GHS Symbols:
GHS05
GHS06
H Phrases:
H301
H314
P Phrases:
P280
P305+P351+P338
P310
EINECS:
206-350-2
CS:
29049900
Download TDS file for complete specifications