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Coomassie® Brilliant Blue R-250 (C.I. 42660)

Code
A1092
CAS
6104-59-2
Molecular Formula
C45H44N3NaO7S2
Molar mass
825.98 g/mol

Recommended prices only. To see your prices login or contact your local distributor.
Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A1092,0010
code
A1092,0010
packaging size
10 g
Product discontinued. Alternative A1092,0025 (please check specifications).
A1092,0025
code
A1092,0025
packaging size
25 g
price per unit
single $42,75
box price per unit
A1092,0100
code
A1092,0100
packaging size
100 g
price per unit
single $121,05
box price per unit
Physical Description:
Solid
Product Code:
A1092
Product Name:
Coomassie® Brilliant Blue R-250 (C.I. 42660)
Headline Comment:
® registered trademark of Imperial Industries PLC
Specifications:
E 1 %,1 cm, λmax.: >300 (pH 7.0)
λmax. (buffer pH 7.0): 554 - 563 nm
Comment:
Coomassie® Brilliant Blue R-250 is one of the most commonly used stains for proteins, after their separation by polyacrylamide gel electrophoresis. The protein-dye complex has an absorption maximum at 549 nm, the dye without protein at 555 nm (in 0.01 M citrate buffer, pH 3). The intensity in staining of proteins probably depends on the basicity of a protein (5). Per positively charged amino acid approximately 1.5 - 3 molecules of Coomassie® will be bound. This variation complicate the exact protein determination with albumin as a standard, since this protein contains more basic amino acids than many other proteins (5).There do exist many protocols for sensitive staining procedures with Coomassie® (e. g. ref. 3, 4). The sensitivity reaches a limit at 25 ng protein (4). We recommend the following protocol\:I. Staining solution\: 0.1 % Coomassie® Brilliant Blue R-250 (Prod.-No. A1092)20 % methanol (or ethanol)10 % acetic acidThe SDS gel (without 'stacking gel') is stained for 1 hour at 60°C or for 2 hours at 50°C or over night at RT.II. Destaining solution\: 20 % methanol (or ethanol)10 % acetic acidDestain the gel for 3 - 4 hours at 50 - 60°C. Add some sponges.Subsequently wash the gel for 15 minuts in water and dry under vacuum at 60°C for 2 - 3 hours.
Bibliography:
(1)Fazekas De St. Groth, S. et al. (1963) Biochim. Biophys. Acta 71, 377-391Two new staining procedures for quantitative estimation of proteins on electrophoresis strips. (2)Chrambach, A. et al. (1967) Anal. Biochem. 20, 150-154A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis. (3)Neuhoff, V. et al. (1988) Electrophoresis 9, 255-262Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie® Briliant Blue G-250 and R-250. (4)Choi, J.-K. et al. (1996) Anal. Biochem. 236, 82-84Modified Coomassie® Blue staining of proteins in polyacrylamide gels with Bismark brown. (5)Tal, M. et al. (1985) J. Biol. Chem. 260, 9976-9980Why does Coomassie® Brilliant Blue R Interact Differently with Different Porteins?
WGK:
1
Storage:
RT
EINECS:
228-060-5
CS:
32041200
Download TDS file for complete specifications