Packs sizes (2)
| code | packaging size | price per unit | box price per unit | |
|---|---|---|---|---|
| Code & packaging | Price per piece | |||
|
code
A1092,0025
|
packaging size
25 g
|
price per unit
single
$45,00
|
box price per unit
|
|
code
A1092,0100
|
packaging size
100 g
|
price per unit
single
$127,20
|
box price per unit
|
Technical data
- Physical Description:
- Solid
- Product Code:
- A1092
- Product Name:
- Coomassie® Brilliant Blue R-250 (C.I. 42660)
- Headline Comment:
- ® registered trademark of Imperial Industries PLC
- Specifications:
- E 1 %,1 cm, λmax.: >300 (pH 7.0)
λmax. (buffer pH 7.0): 554 - 563 nm
- Comment:
Coomassie® Brilliant Blue R-250 is one of the most commonly used stains for proteins, after their separation by polyacrylamide gel electrophoresis. The protein-dye complex has an absorption maximum at 549 nm, the dye without protein at 555 nm (in 0.01 M citrate buffer, pH 3). The intensity in staining of proteins probably depends on the basicity of a protein (5). Per positively charged amino acid approximately 1.5 - 3 molecules of Coomassie® will be bound. This variation complicate the exact protein determination with albumin as a standard, since this protein contains more basic amino acids than many other proteins (5). There do exist many protocols for sensitive staining procedures with Coomassie® (e. g. ref. 3, 4). The sensitivity reaches a limit at 25 ng protein (4). We recommend the following protocol\: I. Staining solution\: 0.1 % Coomassie® Brilliant Blue R-250 (Prod.-No. A1092) 20 % methanol (or ethanol) 10 % acetic acid The SDS gel (without 'stacking gel') is stained for 1 hour at 60°C or for 2 hours at 50°C or over night at RT. II. Destaining solution\: 20 % methanol (or ethanol) 10 % acetic acid Destain the gel for 3 - 4 hours at 50 - 60°C. Add some sponges. Subsequently wash the gel for 15 minuts in water and dry under vacuum at 60°C for 2 - 3 hours.
- Bibliography:
(1) Fazekas De St. Groth, S. et al. (1963) Biochim. Biophys. Acta 71, 377-391 Two new staining procedures for quantitative estimation of proteins on electrophoresis strips. (2) Chrambach, A. et al. (1967) Anal. Biochem. 20, 150-154 A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis. (3) Neuhoff, V. et al. (1988) Electrophoresis 9, 255-262 Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie® Briliant Blue G-250 and R-250. (4) Choi, J.-K. et al. (1996) Anal. Biochem. 236, 82-84 Modified Coomassie® Blue staining of proteins in polyacrylamide gels with Bismark brown. (5) Tal, M. et al. (1985) J. Biol. Chem. 260, 9976-9980 Why does Coomassie® Brilliant Blue R Interact Differently with Different Porteins?
- WGK:
- 1
- Storage:
- RT
- EINECS:
- 228-060-5
- CS:
- 32041200
