Rhodamine B (C.I. 45170)

Assay (photometr.): min. 90 %

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	code	packaging size	price per unit	box price per unit
	Code & packaging		Price per piece
	code A3930,0100	packaging size 100 g	price per unit single $71,10	box price per unit

Product Code:: A3930
Product Name:: Rhodamine B (C.I. 45170)
Specifications:: Assay (photometr.): min. 90 %
λ_max. (50 % EtOH): 550 - 552 nm
E 1 %/1 cm, λ_max.: 2115 - 2350
pH (0.5 %; H₂O; 20°C): approx. 2.0
Loss on drying: max. 5 %
UV/VIS spectrum: passes test
Comment:: The mixed-dye staining method of proteins in polyacrylamide gels with Eriochrome black T in combination with Rhodamine B can detect as little as 10 ng of BSA within one hour (1) and is more sensitive than Coomassie®-staining. The optimum dye concentration of Eriochrome Black T was determined to be 0.01 %. Protein bands were scanned at 560 nm by densitometer. Rhodamine B was employed at the same concentration (0.01 %). Staining solution was prepared by mixing stock solutions of Eriochrome Black T (0.02 % (w/v)) and Rhodamine B (0.02 % (w/v)) in 40 % methanol/7 % acetic acid in a ratio of 1 : 1 (v/v) just prior to use. Besides sensitivity, another advantage of the mixed-dye staining method over Coomassie® is the stability of the staining pattern after drying (60°C, 30 min; ref. 1).
Bibliography:: (1)Jung, D.-W. et al. (1998) Anal. Biochem. 263, 118-120Detection of Proteins in Polyacylamide Gels Using Eriochrome Black T and Rhodamine B.