Packs sizes (1)
code | packaging size | price per unit | box price per unit | |
---|---|---|---|---|
Code & packaging | Price per piece | |||
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code
A3930,0100
|
packaging size
100 g
|
price per unit
single
$92,70
|
box price per unit
|

Technical data
- Solubility:
- 34 g/L (H2O)
- Physical Description:
- Solid
- Product Code:
- A3930
- Product Name:
- Rhodamine B (C.I. 45170)
- Specifications:
- Assay (photometr.): min. 90 %
λmax. (50 % EtOH): 550 - 552 nm
E 1 %/1 cm, λmax.: 2115 - 2350
pH (0.5 %; H2O; 20°C): approx. 2.0
Loss on drying: max. 5 %
UV/VIS spectrum: passes test
- Comment:
The mixed-dye staining method of proteins in polyacrylamide gels with Eriochrome black T in combination with Rhodamine B can detect as little as 10 ng of BSA within one hour (1) and is more sensitive than Coomassie®-staining. The optimum dye concentration of Eriochrome Black T was determined to be 0.01 %. Protein bands were scanned at 560 nm by densitometer. Rhodamine B was employed at the same concentration (0.01 %). Staining solution was prepared by mixing stock solutions of Eriochrome Black T (0.02 % (w/v)) and Rhodamine B (0.02 % (w/v)) in 40 % methanol/7 % acetic acid in a ratio of 1 : 1 (v/v) just prior to use. Besides sensitivity, another advantage of the mixed-dye staining method over Coomassie® is the stability of the staining pattern after drying (60°C, 30 min; ref. 1).
- Bibliography:
(1) Jung, D.-W. et al. (1998) Anal. Biochem. 263, 118-120 Detection of Proteins in Polyacylamide Gels Using Eriochrome Black T and Rhodamine B.
- Hazard pictograms
-
- WGK:
- 2
- Storage:
- RT
- Signal Word:
- Danger
- GHS Symbols:
- GHS05
GHS07
- H Phrases:
- H302
H318
H412
- P Phrases:
- P273
P280
P301+P312
P305+P351+P338
P310
P330
- EINECS:
- 201-383-9
- CS:
- 32041300