Back

Rhodamine B (C.I. 45170)

Assay (photometr.): min. 90 %
Code
A3930
CAS
81-88-9
Molecular Formula
C28H31ClN2O3
Molar mass
479.02 g/mol

Recommended prices only. For prices and orders please contact your local distributor.
Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A3930,0100
code
A3930,0100
packaging size
100 g
price per unit
single $152,55
box price per unit
molecule for: Rhodamine B (C.I. 45170)
Solubility:
34 g/L (H2O)
Physical Description:
Solid
Product Code:
A3930
Product Name:
Rhodamine B (C.I. 45170)
Specifications:
Assay (photometr.): min. 90 %
λmax. (50 % EtOH): 550 - 552 nm
E 1 %/1 cm, λmax.: 2115 - 2350
pH (0.5 %; H2O; 20°C): approx. 2.0
Loss on drying: max. 5 %
UV/VIS spectrum: passes test
Hazard pictograms
  • GHS05 Hazard
  • GHS07 Hazard
WGK:
2
Storage:
RT
Signal Word:
Danger
GHS Symbols:
GHS05
GHS07
H Phrases:
H302
H318
H412
P Phrases:
P280
P301+P312
P305+P351+P338
P310
P330
P501
EINECS:
201-383-9
CS:
32041300
Download TDS file for complete specifications

Comments

The mixed-dye staining method of proteins in polyacrylamide gels with Eriochrome black T in combination with Rhodamine B can detect as little as 10 ng of BSA within one hour (1) and is more sensitive than Coomassie®-staining. The optimum dye concentration of Eriochrome Black T was determined to be 0.01 %. Protein bands were scanned at 560 nm by densitometer. Rhodamine B was employed at the same concentration (0.01 %). Staining solution was prepared by mixing stock solutions of Eriochrome Black T (0.02 % (w/v)) and Rhodamine B (0.02 % (w/v)) in 40 % methanol/7 % acetic acid in a ratio of 1 : 1 (v/v) just prior to use. Besides sensitivity, another advantage of the mixed-dye staining method over Coomassie® is the stability of the staining pattern after drying (60°C, 30 min; ref. 1).

Literature

(1) Jung, D.-W. et al. (1998) Anal. Biochem. 263, 118-120. Detection of Proteins in Polyacylamide Gels Using Eriochrome Black T and Rhodamine B.