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Spermine BioChemica

Assay (titr.): min. 98 %
Code
A0672
CAS
71-44-3
Molecular Formula
C10H26N4
Molar mass
202.34 g/mol

Recommended prices only. For prices and orders please contact your local distributor.
Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A0672,0025
code
A0672,0025
packaging size
25 g
price per unit box price per unit
A0672,1000
code
A0672,1000
packaging size
1 kg
price per unit box price per unit
Physical Description:
Solid
Product Code:
A0672
Product Name:
Spermine BioChemica
Specifications:
Assay (titr.): min. 98 %
pH (1 M; H2O; 20°C): 11.0 - 13.0
Spermidine: max. 0.5 %
Water: max. 1.0 %
Carbonate: max. 0.5 %
Hazard pictograms
  • GHS05 Hazard
UN:
3259
Class/PG:
8/II
ADR:
8/II
IMDG:
8/II
IATA:
8/II
WGK:
1
Storage:
2 - 8°C
Signal Word:
Danger
GHS Symbols:
GHS05
H Phrases:
H314
P Phrases:
P280
P303+P361+P353
P305+P351+P338
P310
P321
P405
P501
EINECS:
200-754-2
CS:
29212900
Download TDS file for complete specifications

Comments

Spermine belongs to the Polyamines. When precipitating selectivly DNA with the help of Spermine most of the proteins and triphosphates will not coprecipitate. The precipitation with spermine is applied after restriction digests, the separation of DNA fragments and triphosphates and the purification of DNA fragments after PAGE. Stock solutions are prepared with a concentration of 100mM (pH 6.8, ref. 2) and stored at +4°C (short-term) or at -20°C (long-term). The working concentration is approx 0.1 mM, if the DNA is dissolved in TE.

Literature

(1) Blumenthal, A.B. et al. (1979) J. Cell Biol. 81, 255-259. Rapid isolation of Metaphase chromosomes containing high molecular weight DNA. (2) Hoopes, B. & McClure, W. (1981) Nucleic Acids Res. 9, 5493-5504. Studies on the selectivity of DNA precipitation by spermine. (3) Tabor, C.W. & Tabor, H. (1984) Ann. Rev. Biochem. 53, 749-790. Review article: Polyamines. (4) Cram, L.S. et al. (1990) Methods Cell Biol. 33, 377-382. Polyamine buffer for bivariate human flow cytogenetic analysis and sorting.