Back

BCIP BioChemica

Assay (HPLC): min. 98 %
Code
A1117
CAS
6578-06-9
Molecular Formula
C8H6BrClNO4P · C7H9N
Molar mass
433.64 g/mol

Recommended prices only. To see your prices login or contact your local distributor.
Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A1117,0500
code
A1117,0500
packaging size
500 mg
price per unit
single 80,50€
box price per unit
A1117,0001
code
A1117,0001
packaging size
1 g
price per unit
single 129,00€
box price per unit
A1117,0005
code
A1117,0005
packaging size
5 g
price per unit
single 469,40€
box price per unit
Solubility:
20 mg/mL (DMF)
Physical Description:
Solid
Product Code:
A1117
Product Name:
BCIP BioChemica
Specifications:
Assay (HPLC): min. 98 %
λmax. (buffer pH 7.0): 286 - 294 nm
Water (K.F.): max. 1 %
Hazard pictograms
  • GHS07 Hazard
  • GHS08 Hazard
WGK:
1
Storage:
-20°C
protected from light
Signal Word:
Attention
GHS Symbols:
GHS07
GHS08
H Phrases:
H302
H315
H319
H335
H371
P Phrases:
P260
P280
P301+P312
P305+P351+P338
P405
P501
EINECS:
229-506-1
CS:
29339980
Download TDS file for complete specifications

Comments

BCIP (X-Phos p-Toluidine salt) is a substrate of the alkaline phosphatase, which releases the blue stain 5-Bromo-4-chloro-3-indoxyl (X) by cleaving the substrate. The enzymatically released indoxyl will be oxidized by NBT (A1243) to reveal the insoluble indigo. Depending on the author, BCIP is dissolved in anhydrous dimethylformamide (DMF) or DMSO in concentrations ranging from 5 to 50 mg/ml and stored at -20°C or \+4°C. Solutions of BCIP are stable for approx. 1 year at \+4°C.

Literature

(1)Horwitz, J.P. et al. (1966) J. Med. Chem. 9, 447Substrate for cytohistochemical determination of enzyme activities\: Dihalo-3-indolylphosphates. (2)Gossrau, R. (1978) Histochemistry 58, 203-218Comparison of the sensitivity of different hydrolase substrates for their histochemical determination. (3)Leary, J.J. et al. (1983) Proc. Natl. Acad. Sci. USA 80, 4045-4049Bio-blots for DNA and RNA with BCIP as a substrate/phosphatase linked antibody. (4)Ey, P.L. & Ashman, L.K. (1986) Methods Enzymol. 121, 497-509Determination of alkaline phosphatase connected antibodies in immunoblots. (5)Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology. Supplement 66, Page 10.8.17. Greene Publishing & Wiley-Interscience, New York.