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Albumin (BSA) Fraction V (pH 7.0)

Code
A1391
CAS
9048-46-8
Molar mass
approx. 68000 g/mol

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code packaging size price per unit box price per unit
Code & packaging Price per piece
A1391,0025
code
A1391,0025
packaging size
25 g
price per unit
single 63,00€
box price per unit
A1391,0050
code
A1391,0050
packaging size
50 g
price per unit
single 102,70€
box price per unit
A1391,0100
code
A1391,0100
packaging size
100 g
price per unit
single 184,30€
box price per unit
A1391,0250
code
A1391,0250
packaging size
250 g
price per unit
single 338,00€
box price per unit
A1391,0500
code
A1391,0500
packaging size
500 g
price per unit
single 612,80€
box price per unit
A1391,1000
code
A1391,1000
packaging size
1 kg
price per unit
single 1026,10€
box price per unit
Physical Description:
Solid
Product Code:
A1391
Product Name:
Albumin (BSA) Fraction V (pH 7.0)
Specifications:
Proteases: not detectable
Purity Albumin (Electrophoresis): min. 98 %
Mesophilic germs: max. 100 CFU/g
pH (2 %; H2O; 20°C): 6.6 - 7.5
Heavy metals: max. 0.001 %
Loss on drying (105°C; 4 h): max. 3 %
WGK:
1
Storage:
2 - 8°C
Origin:
from bovine serum
EINECS:
232-936-2
CS:
35029070
Download TDS file for complete specifications

Comments

Bovine serum albumin (BSA) is added as a stabilizing component for proteins / enzymes to several enzyme reaction and storage buffers. The concentration usually ranges from 0.01 % (0.1 mg/ml; e. g. ref. 2) to 3 % (30 mg/ml; e. g. ref. 1, 2). BSA is added to the 10X concentrated buffers of DNA-modifying enzymes or restriction enzymes in a concentration of 0.5 mg/ml (see e. g. ref. 6, 8). Alternatively, BSA can be substituted by gelatin for such purposes at the same concentration. Besides, albumin is applied as a blocking agent for blocking unbound surfaces of blotting membranes in immunoblots (3 %; ref. 1, 2, 7) or ELISAs (3 % in PBS, ref. 2) or for the dilution of antisera and antibody-stock solutions, respectively. In ELISAs, BSA is frequently replaced by non-fat dried milk (A0830). As standard for protein determinations see ref. 9.This fraction of albumin has been manufactured by a combination of the heat-shock method and alcohol precipitation. Albumin is stable as powder (3 years) or in solution (biological buffers like PBS; one year at \+4°C to -20°C). Stock solutions are prepared in concentrations up to 20 %. If crystals are formed during storage of the solutions, they can be redissolved by warming up to 37°C and mixing. Usually, sodium azide is added at a final concentration of 2 mM (or 0.02 - 0.2 %) to prevent microbial contaminations.

Literature

(1)Taylor, J.A. et al. (1995) Mol. Cell. Biol. 15, 4149-4157Application\: Blocking of free surfaces of immuno blots. (2)Reinhard, M. et al. (1995) EMBO J. 14, 1583-1589Application\: Blocking of free surfaces of immuno blots. (3)O'Neill, S.D. & Spanswick, R.M. (1984) J. Membrane Biol..79, 231-2439Stabilization of proteins during homogenization of plant tissue. (4)Fazekas de St. Groth, S. et al. (1963) Biochim. Biophys. Acta 71, 377-391Standard for calibration curves for the determination of the protein concentration. (5)Peters, T.A. & Sjöholm, I. eds. (1977) Albumin\: Structure, Biosynthesis, Function. FEBS 11th Meeting Copenhagen 1977 Vol. 50 Colloquium B9 [Pergamon Press]. (6)Cobianchi, F. & Wilson, S.H. (1987) Methods Enzymol. 152, 94-110.Enzyme for the modification and labeling of DNA and RNA. (7)Harlow. E. & Lane, D. eds. (1988) Antibodies\: A Laboratory Manual. Cold Spring Harbour (Seite 496\+684). (8)Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. eds. (2001) Current Protocols in Molecular Biology. Supplement 21, Page 3.4.2, Greene Publishing & Wiley-Interscience, New York. (9)Bradford, M.M. (1976) Anal. Biochem. 72, 248-254Quantification of protein concentration in microgram-quantities.