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IPTG BioChemica

Assay (HPLC): min. 99 %
Code
A1008
CAS
367-93-1
Molecular Formula
C9H18O5S
Molar mass
238.30 g/mol

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Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A1008,0005
code
A1008,0005
packaging size
5 g
price per unit
single 95,40€
box price per unit
A1008,0025
code
A1008,0025
packaging size
25 g
price per unit
single 399,50€
box price per unit
A1008,0050
code
A1008,0050
packaging size
50 g
price per unit
single 695,80€
box price per unit
A1008,0100
code
A1008,0100
packaging size
100 g
price per unit
single 1234,60€
box price per unit
Physical Description:
Solid
Product Code:
A1008
Product Name:
IPTG BioChemica
Specifications:
Assay (HPLC): min. 99 %
α20°C/D; 1 %, H2O: -28.5° - -34.5°
pH (5 %; H2O): 5.0 - 7.0
Dioxane: max. 0.0001 %
Water (K.F.): max. 1.0 %
WGK:
1
Storage:
2 - 8°C
protected from light
Shipment:
ambient temperature
EINECS:
206-703-0
CS:
29329900
Download TDS file for complete specifications

Comments

The lactose-operon from E. coli is under negative control of the Lac-repressor protein, which specifically binds to the operator of the E. coli-genom. The most commonly used synthetic inductor of the Lac-operon is IPTG since it is both active at very low concentrations and not subject to enzymatic degradation. The final concentration differs from author to author (0.1 mM (2) - 1 mM (1) IPTG in LB-medium). For preparing a stock solution dissolve 100 mM or 500 mM IPTG in water or buffer, sterilize by filtration through a 0.22 μm filter and store at -20°C.

Literature

(1)Yansura, D.G. & Henner, D.J. (1984) Proc. Natl. Acad. Sci. USA 81, 439-443Use of the E.coli lac repressor and operator to control gene expression in B. subtilis. (2)Cho, S. et al. (1985) Biochem. Biophys. Res. Com. 128, 1268-1273Effect of IPTG concentration on the level of Lac-Operon induction in steady state E. coli.